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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two further splice variants have been reported but in depth nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind particularly for the target antibody and block antibody binding. These peptide normally consists of the epitope identified by the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to be sure the absolute best results are...
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This manual outlines more attainable optimization of mobile and immunoassay parameters to make sure the best possible success are...
Antibodies bound click here to the blocking peptide no more bind for the epitope around the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Goal Information and facts Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic party. Cyclin E varieties a fancy with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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